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Estrogenic and anti-neutrophilic inflammatory phenanthrenes from Juncus effusus L.

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journal contribution
posted on 2021-09-09, 13:05 authored by Hao-Chun Hu, Yi-Hong Tsai, Yu-Che Chuang, Kuei-Hung Lai, Yu-Ming Hsu, Tsong-Long Hwang, Chih-Chan Lin, Ferenc Fülöp, Yang-Chang Wu, Szu-Yin Yu, Yu-Ting Kuo, Fang-Rong Chang

Juncus effusus L. (J. effusus) is a Traditional Chinese Medicine (TCM) that has long been used for dealing with gynaecological disorders, such as relieving insomnia, preventing tinnitus, reducing edema with diuretic effect. In our course of evidence-based medical research focused on this herb, one new phenanthrene, Junfusol B (2), together with seventeen known compounds were isolated and identified. All the structures of these compounds were elucidated by spectroscopic methods. The absolute stereochemistry of compounds 1 and 2 was further determined by comparing their calculated and experimental Electronic Circular Dichroism (ECD) spectra and optical rotation (OR) values. The isolates were evaluated for their estrogenic and anti-inflammatory activities which were considered as relevant etiological factors of insomnia, tinnitus and edema in the ancient TCM theory. The results revealed that most of the obtained phenanthrenes in this work were found exerting agonistic effects on estrogen receptor. This is the first report to declare the exact estrogen-regulating potential among this type of compounds from J. effusus. Moreover, phenanthrenes 3  7 exhibited significant inhibitions on superoxide anion generation and elastase release in fMLP/CB-induced human neutrophilic inflammation model. J. effusus may be developed as a complementary agent utilized in menopausal multiple syndromes.

Funding

This research was funded by the Ministry of Science and Technology, Taiwan awarded to F.-R. Chang, grant number: MOST 105-2628-B-037-001-MY3, MOST 106-2320-B-037-008-MY2, MOST 106-2911-I-037-504, MOST 108-2320-B-037-022-MY3, 109-2811-B-037-517, and 109-2927-I-037-502. In addition, this research was partially funded by the Drug Development and Value Creation Research Center of Kaohsiung Medical University; Department of Medical Research of Kaohsiung Medical University Hospital awarded to F.-R. Chang (grant number: KMU-TC108A03-11). Moreover, this research was funded by the Chi Mei Medical Center and Kaohsiung Medical University awarded to Y.-T. Kuo, grant number: 103-CM-KMU-13. We would like to specially thank the Center for Research Resources and Development (CRRD) of Kaohsiung Medical University for providing the assistance in NMR, LC-MS and GC-MS.

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