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New compounds from the stems of Fissistigma acuminatissimum Merr. and their in vitro anti-inflammatory activity

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journal contribution
posted on 14.05.2022, 12:20 by Chen Jin, Ling Zhang, Cheng-Jun Yuan, Yi Liu, Hui-Zhen Liu, Bo-Lun Xu, Ji-Cheng Shu, Xiao-Yu Zhang, Si-Yi Wang, Hui-Lian Huang

Three new compounds, 4,5,6,7-tetramethoxy-3-benzoylbenzofuran (1), 4-hydroxy-3,5,6-trimethoxydihydrochalcone-2-O-β-D-glucopyranoside (2) and 2-hydroxy-3,4,5,6-tetramethoxyphenylethyl benzoate (3) along with five known flavonoids were isolated from the dichloromethane fraction of the stems of Fissistigma acuminatissimum Merr.’s ethanol extracts. The compounds were obtained by chromatographic methods and the structure elucidation was completed primarily on the basis of spectroscopic analyses, all of these compounds were isolated from F. acuminatissimum for the first time. All the fractions and compounds were evaluated for their anti-inflammatory activity against lipopolysaccharide (LPS)-stimulated tumor necrosis factor α (TNF-α) production in RAW264.7 cells in vitro. The dichloromethane fraction showed the most potent inhibition(38.2%) at 60 μg/mL, compound 1 (70.2%) and 3 (65.2%) showed significant inhibition at 10 μM.

Funding

This work was financially supported by the Academic and Technical Leader Program of Jiangxi Province under Grant 20172BCB22010, the National Natural Science Foundation of China under Grant 81960697 & 82160792, Natural Science Foundation of Jiangxi Province under Grant 20192ACB20016, Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program, the Young Teacher Training Plan for Key Subjects of JXUCM under Grant 2021jzzdxk003.

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