Mesoporous silica particles are phagocytosed by microglia and induce a mild inflammatory response in vitro nnm-2022-0026 Suppl File 1
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posted on 2022-08-23, 10:41 authored by Paula Carreras-Dom´ınguez, Chunfang Zhou, Neus Rabaneda-Lombarte, Carme Solà, Jose M Vidal-Taboada, Adam Feiler, Ninnie Abrahamsson, Elena N Kozlova, Josep Saura, Júlia Sala-Jarque, Elisa Garcıa-Lara<p>Aim: Mesoporous silica particles (MSPs) are broadly used drug delivery carriers. In this study, the</p>
<p>authors analyzed the responses to MSPs of astrocytes and microglia, the two main cellular players in</p>
<p>neuroinflammation. Materials & methods: Primary murine cortical mixed glial cultures were treated with</p>
<p>rhodamine B-labeled MSPs. Results: MSPs are avidly internalized by microglial cells and remain inside the</p>
<p>cells for at least 14 days. Despite this, MSPs do not affect glial cell viability or morphology, basal metabolic</p>
<p>activity or oxidative stress. MSPs also do not affect mRNA levels of key proinflammatory genes; however,</p>
<p>in combination with lipopolysaccharide, they significantly increase extracellular IL-1β levels. Conclusion:</p>
<p>These results suggest that MSPs could be novel tools for specific drug delivery to microglial cells.</p>
