New class of diethyl substituted phosphoramidimidates and phosphonimidates: synthesis, spectral characterization and antimicrobial activity

<p>A series of new class of diethyl <i>N</i>-2-hydroxyethyl-<i>N'</i>-substituted phosphoramidimidates <b>6(a–e)</b> and diethyl <i>P</i>-morpholino-<i>N</i>-substituted phosphonimidates <b>6(f–j)</b> was synthesized. The precursor intermediates, diethyl substituted phosphoramidites <b>3(a–b)</b> were prepared initially by a reaction of various amines <b>1(a–b)</b> and diethyl phosphorochloridite <b>(2)</b> and then they were treated by <i>in situ</i> with aromatic/alkyl azides through Staudinger reaction to accomplish title products. Structures of all the synthesized compounds were characterized by spectroscopic data such as IR, NMR (<sup>1</sup>H, 13C, <sup>31</sup>P), mass, and elemental analyses. The synthesized compounds were screened for their <i>in vitro</i> antimicrobial activity to understand their biological potency. The biological screening results disclosed that compounds <b>6b, 6c, 6e, 6g, 6h and 6j</b> having potent antimicrobial activity against all the tested pathogens.</p>