Process optimization for effective bio-decolourization of reactive orange 16 using chemometric methods

Azo group containing reactive dyes are most commonly used in textile and tannery industries due to its bright appearance and stable color. This study aims to investigate the decolourization of reactive orange 16 (RO16) dye by Pseudomonas aeruginosa 23N1 along with removal of chromate (Cr(VI)) and evaluation of optimal process condition. The regular two-level factorial design is used to screen out operational parameters and selects their levels for further optimization process through central composite design (CCD) based response surface methodology (RSM). The result revealed that glucose and peptone have a negative effect on the performance of dye decolourization. Bacteria exhibit high decolourization potential in yeast extract supplemented culture medium with no addition of external carbon sources. The percentages of decolourization obtained in model validated experiments are obtained as 95.0 ± 0.4% and 95.1 ± 0.5% for initial dye 50 mg/L and 150 mg/L, respectively, which exhibit satisfactory correlation with model predicted response. The simultaneous dye and Cr(VI) removal has been explored in this study. The decolourization of dye is only affected due to presence of high Cr(VI) concentration (>120 mg/L). Bacteria have shown satisfactorily decolourization for RO16 contaminated industrial wastewater. The strain 23N1 could be a good biological agent for decolourization of RO16 dye.