The mixed-species biofilm of Lactobacillus plantarum ML11-11 (LAB) and yeast had a double-layered structure with the ground layer composed of LAB cells, and the upper layer composed of coaggregates of LAB and yeast cells. The ability of LAB to adhere to both, the solid surface and the yeast cells, enabled the formation and maintenance of the biofilm as an ecosystem for LAB and yeast.
Funding
This work was supported by the High-Tech Research Center Project (grant no. 07H015, the M. Nakajima project) for Private Universities, with a matching fund subsidy from MEXT (Ministry of Education, Culture, Sports, Science, and Technology, Japan).