Influence of chitosan-tripolyphosphate nanoparticles on thermosensitive polymeric hydrogels: structural organization, drug release mechanisms and cytotoxicity

Mariano, Kelli C. Freitas; Nascimento, Mônica H. Monteiro do; Querobino, Samyr M.; Campos, Estefânia V. Ramos; de Oliveira, Jhones L.; Yokaichiya, Fabiano; Franco, Margareth K.K.D.; Alberto-Silva, Carlos; de Paula, Eneida; Lombello, Christiane B.; de Lima, Renata; Fraceto, Leonardo F.; de Araujo, Daniele R.

doi:10.6084/m9.figshare.7991486.v2

gpom_a_1596909_sm0760.docx (228.72 kB)

Influence of chitosan-tripolyphosphate nanoparticles on thermosensitive polymeric hydrogels: structural organization, drug release mechanisms and cytotoxicity

Version 2 2020-02-25, 09:19

Version 1 2019-04-13, 04:45

journal contribution

posted on 2020-02-25, 09:19 authored by Kelli C. Freitas Mariano, Mônica H. Monteiro do Nascimento, Samyr M. Querobino, Estefânia V. Ramos Campos, Jhones L. de Oliveira, Fabiano Yokaichiya, Margareth K.K.D. Franco, Carlos Alberto-Silva, Eneida de Paula, Christiane B. Lombello, Renata de Lima, Leonardo F. Fraceto, Daniele R. de Araujo

Chitosan-tripolyphosphate (CS-TPP) nanoparticles containing naproxen (NPX) were dispersed in poloxamer (PL) as unique (PL407) or binary (PL407-PL403) systems. Nanoparticles presented diameter of ∼250 nm and zeta potential of ∼35 mV with drug loading and encapsulation efficiency of 98.4 ± 0.3% and 36.9 ± 0.12%, respectively. NPX-CS-TPP shifted the sol-gel transition and micellization temperatures. PL407-PL403 systems presented G′ > G″ compared to PL407. SAXS patterns revealed transitions from lamellar to hexagonal phase organizations with low drug release rates, in the presence of CS-TPP nanoparticles. NPX-CS-TPP-PL407 induced lower cytotoxicity compared to PL407-PL403 in fibroblasts and osteoblasts, making them promising systems for intra-articular delivery.